Nope. This wasn't focus stacked. There was enough light to get it up to 6.3. It seems like f8 is where to be for good dof for bugs (so far). I'm just lucky that the robber flies are so incredibly interesting. My other bug shots didn't go over as well. But it's been fun. I need to play with this more often. :)
You are a master of all types of photography. I have that lens but have not come even a million miles near capturing anything as superbly as this. Out of interest is there any focus stacking or a single shot?