ISO 3200
1s shutter speed
f/6.3
Lens at 400mm
Image stabilization turned off
Shutter release
Shooting from live view so no lens movement at all
Long exposure noise reduction enabled
Processed in Topaz DeNoise to knock the noise down a bit, and Nik Sharpener Efex Pro to sharpen it back up after the noise reduction (I love that combination for sharp/low noise images).