Shot with my 150 with a 50 reversed on the end. This guy is VERY, VERY small. This is a heavily cropped 3:1 view, I got another that was with a reversed 24mm on the end, but the DoF wasn't as good and he moved while I was trying to setup the multiple shots for focus stacking (he didn't like the high powered flashes from the ABR800). If this does well at all I'll post the scale shot so you can get an idea of his size.
No sedation/cooling needed- he just liked hanging out on the rim.
Here's an image for scale-
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I really hate bugs - gives me the heebie-jeebies. However this is one heck of an awesome capture - especially for the size! All the detail makes it even more creepy. :D